Chemical Composition and Biological Activities of Essential Oil from Citrus Sinensis Peel

The present study was aimed to evaluate the in vitro thrombolytic, anti-haemolytic and anti-inflammatory activities from essential oil of C. sinensis peel. C. sinensis collected and was subjected to hydrodistillstion by using Clevenger apparatus. The identification of compounds present in the oil of C. sinensis was carried out by gas chromatography and mass spectrometry (GC-MS). In vitro thrombolytic, hemolytic and anti-inflammatory activities were studied by standard protocols. From the GC-MS report it has been estimated that 10 major compounds present in the essential oil. The essential oil of C. sinensis shows potent thrombolytic activity and the percentage of inhibition were found to be 90±1.65% at 100 μL. In hemolytic activity, the percentage of inhibition of C. sinensis is about 68%. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay. The results showed that essential oil of C. sinensis peel at a concentration range of 100 μl significantly protects the heat induced protein denaturation at 69%. The results obtained in the present study indicate that essential oil of C. sinensis peel can be a potential source of thrombolytic, anti-haemolytic and anti-inflammatory agents.